With the growth of different industries that depend on biometric and chemical advancements, there is higher emphasis on accuracy and reliability of results. Products that are developed commercially need to have a high level of purity, and in view of this, products such as chromatography paper have found their way into modern chromatography.
These filtration papers do more than just separate particles ‚Äì they are manufactured with precision to ensure that the highest degree of quality, purity and consistency are always maintained when they are put to use. There are variants of the standard types of chromatography paper, and identifying the right one for you depends on the samples on which you are working.
Ion Exchange Papers
Not all filtration samples involve just are aqueous or chemical-based separation. At times, your research could involve more precise separation of particles. These papers are ideal for two-dimensional chromatography and are used in sample separation based on polarity or charge.
This type of chromatography paper comes in different grades, and each of these grades caters to specific ion exchange capacities, flow rates and filtration capabilities. These papers are also ideal when multiple solvents are being used on the same sample either sequentially or simultaneously.
Some of the common applications in which ion exchange papers are also used include DNA polymerase, reverse transcriptase assays, protein kinase assays with peptide substrates and also the separation of phenols, dyes, phospholipids and steroids. The media that is used for these papers is either fibrous or micro-granular.
Cellulose Chromatography Paper
Made from pure cellulose, these papers for chromatography are used in different chromatography techniques. Used widely in nucleic acid and enzyme blotting, these papers also have uniformity of capillary action and wicking capabilities that are very essential in chemical separation of samples.
Different grades of this type of chromatography paper define which paper would be right under specific circumstances. Choosing the right grade will ensure that you have the right paper for your sample. These grades are based on various factors such as the thickness of the paper, smoothness, pore size, flow rate, treatment and chemical compatibility.
Processes such as analytical separations, optical and radiometric scanning, electrophoresis and separation of organic and inorganic compounds require different types of this paper. Varying grades, thickness and pore size will help you in precise separation. Cellulose papers are also widely used in cases where speed is important, compositions of samples are unknown or large amounts of substance need to be separated.
Importance of the Right Chromatography Products and Papers
High levels of purity and filtration performance are non-negotiable in critical environments such as biotechnological and pharmaceutical industries. Choosing high quality chromatography paper and other chromatography products from Tisch Scientific will help you get the results you need to make your research and development a success.
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